Analysis Note

ControlEye

Application

Western blot: 1:100-1:500 using ECL. Suggested dilution buffer is PBS and blocking buffer is PBS containing 5% milk. Recognizes 36 kDa monomers, dimmers and trimers depending on sample preparation. Boiling induces the aggregation of rhodopsin.

Western blot of isolated bovine rod outer segment labeled for rhodopsin (~85% of rod outer segment protein) with the rho 1D4 antibody. Amount of rod outer segments applied to the SDS gel: (a) 2.5 mg; (b) 0.63 mg; (c) 0.16 mg; (d) 0.04 mg. At higher protein quantities, dimers, trimers and tetramers of rhodopsin can be observed along with the monomer.

Immunohisto/cytochemistry: 1:100-1:500 on paraformaldehyde and glutaraldehyde fixed frozen tissue sections. Preferred fixation is paraformaldehyde for 4 hours at 2-8°C. Suggested permeabilization method is 0.2% Triton X-100.

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Anti-Rhodopsin Antibody, C-terminus, last 9 amino acids, clone Rho 1D4 detects level of Rhodopsin & has been published & validated for use in IC, IH, IP & WB.

Research CategoryNeuroscience

Research Sub CategorySensory & PNS

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Bovine rhodopsin

Epitope: C-terminus, last 9 amino acids

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Specificity

Recognizes Rhodopsin. Reacts with both native and recombinant protein. No known reactivity to other proteins.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

48 kDa